Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Efficient targeted deletion of Stat2 in mouse tissues. Expression of STAT2 and STAT1 was analyzed in various tissues by Western blot analysis. ( a ) Bone marrow–derived cDCs generated from Stat2 fl/fl mice crossed with CD11c-Cre mice ( Stat2 Δ-cDC ). ( b ) Colons from Stat2 fl/fl mice crossed with Cdx2-Cre mice ( Stat2 Δ-CE ). ( c ) Lungs from Stat2 Δ-cDC and Stat2 Δ-CE mice were included for specificity of targeted deletion. Wild-type (WT) and Stat2KO mice served as positive and negative controls, respectively. ACTIN was used as an internal protein loading control.

Article Snippet: The following mouse strains on the B6 background were purchased from The Jackson Laboratory: FLPe deleter (Strain#: 009086), CMV-Cre (Strain#: 006054), CD11c-Cre (Strain#: 008068) and Cdx2-Cre (Strain#: 009350).

Techniques: Expressing, Western Blot, Derivative Assay, Generated, Control

Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Generation of conditional Stat2 KO mice. ( a ) Targeting strategy used to construct Stat2 fl/fl mice with exons 5 through 8 flanked with loxP sites. E represents exons with their corresponding number. Brown boxes depict exons in wild type allele and green boxes depict exons after successful integration of targeting vector. ( b ) Confirmation of loxP sites integration in the Stat2 gene by Southern blot analysis (9.8 kb DNA fragment). ( c ) Genotyping of mouse-tail DNA by PCR confirms deletion of Stat2 floxed allele after breeding with CMV-Cre mouse.

Article Snippet: The following mouse strains on the B6 background were purchased from The Jackson Laboratory: FLPe deleter (Strain#: 009086), CMV-Cre (Strain#: 006054), CD11c-Cre (Strain#: 008068) and Cdx2-Cre (Strain#: 009350).

Techniques: Construct, Plasmid Preparation, Southern Blot

Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Impaired IFN-I signaling after conditional deletion of Stat2 by CMV-Cre—mediated recombination. ( a ) Loss of Stat2 gene expression validated in four different organs by qRT-PCR analysis. ( b ) Western blot analyses performed on several tissues confirm global Stat2 deletion ( Stat2 Δ / Δ ) by ubiquitous CMV-Cre recombinase. ( c ) Splenocytes of the indicated genotypes were left untreated or treated with IFN-β for 6 h, and ISG expression was determined by qRT-PCR. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Data are presented as SEM of three independent experiments. n.s; not significant.

Article Snippet: The following mouse strains on the B6 background were purchased from The Jackson Laboratory: FLPe deleter (Strain#: 009086), CMV-Cre (Strain#: 006054), CD11c-Cre (Strain#: 008068) and Cdx2-Cre (Strain#: 009350).

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing

Journal: Journal of cell science

Article Title: STEAP4 Target NQO1 Mediates Colon Tumorigenesis.

doi: 10.1242/jcs.263402

Figure Lengend Snippet: Fig. 1. Colon Epithelial Cell-Specific Steap4 Knockout Suppresses Mouse Colon Tumor

Article Snippet: Steap4 F/F mice were produced utilizing exon 2~3 of the mouse Steap4 gene as the conditional knockout region at Cyagen (Santa Clara, CA) and crossed with Cdx2 ERT2-Cre Apc F/+ mice to generate Steap4 F/F Cdx2 ERT2-Cre Apc F/+ mice.

Techniques: Knock-Out

Journal: Journal of cell science

Article Title: STEAP4 Target NQO1 Mediates Colon Tumorigenesis.

doi: 10.1242/jcs.263402

Figure Lengend Snippet: Fig. 2. Steap4 Knockdown Suppresses Mouse Colon Tumor Cell Growth In Vitro and In

Article Snippet: Steap4 F/F mice were produced utilizing exon 2~3 of the mouse Steap4 gene as the conditional knockout region at Cyagen (Santa Clara, CA) and crossed with Cdx2 ERT2-Cre Apc F/+ mice to generate Steap4 F/F Cdx2 ERT2-Cre Apc F/+ mice.

Techniques: Knockdown, In Vitro

Journal: Journal of cell science

Article Title: STEAP4 Target NQO1 Mediates Colon Tumorigenesis.

doi: 10.1242/jcs.263402

Figure Lengend Snippet: Fig. 3. STEAP4 Overexpression in Human HCT116 Cells Activates NRF2 Signaling

Article Snippet: Steap4 F/F mice were produced utilizing exon 2~3 of the mouse Steap4 gene as the conditional knockout region at Cyagen (Santa Clara, CA) and crossed with Cdx2 ERT2-Cre Apc F/+ mice to generate Steap4 F/F Cdx2 ERT2-Cre Apc F/+ mice.

Techniques: Over Expression

Journal: Journal of cell science

Article Title: STEAP4 Target NQO1 Mediates Colon Tumorigenesis.

doi: 10.1242/jcs.263402

Figure Lengend Snippet: Fig. 4. Oxidative Stress Response during STEAP4-Catalyzed Ferric Iron Reduction In

Article Snippet: Steap4 F/F mice were produced utilizing exon 2~3 of the mouse Steap4 gene as the conditional knockout region at Cyagen (Santa Clara, CA) and crossed with Cdx2 ERT2-Cre Apc F/+ mice to generate Steap4 F/F Cdx2 ERT2-Cre Apc F/+ mice.

Techniques:

Journal: Journal of cell science

Article Title: STEAP4 Target NQO1 Mediates Colon Tumorigenesis.

doi: 10.1242/jcs.263402

Figure Lengend Snippet: Fig. 5. STEAP4 Overexpression Increases Oxidative Stress and Susceptibility to NQO1

Article Snippet: Steap4 F/F mice were produced utilizing exon 2~3 of the mouse Steap4 gene as the conditional knockout region at Cyagen (Santa Clara, CA) and crossed with Cdx2 ERT2-Cre Apc F/+ mice to generate Steap4 F/F Cdx2 ERT2-Cre Apc F/+ mice.

Techniques: Over Expression

Journal: Nature

Article Title: Sensory input, sex and function shape hypothalamic cell type development

doi: 10.1038/s41586-025-08603-0

Figure Lengend Snippet: a , Distance between mutant and control sample centroids in PCA space (normalized to intra-genotype distance) for excitatory clusters. Control samples included both C57BL/6J and control littermates. Sample numbers, left to right columns, mutant/control: 2/8, 4/10, 2/10, 2/8, 2/8, 2/8, 2/8, 2/7, 2/7, 2/8, 2/8, 2/7, 2/8 and 2/8. cKO, conditional knockout; KO, knockout. b , Mutant–control PCA distance for all clusters in TRPC2 P10 experiments, split according to functional role. c , Pseudotime mapping of Trpc2 mutant (−/−) and control (−/+ littermates) across age. Higher pseudotime values correspond to higher maturity (more similar to adult cell types). Pseudotime is averaged within a cluster; boxplot shows distribution across all cluster averages. P value indicates results from two-sided t -test, mutant versus control cell types. n for increasing age: 82, 137, 131 and 128 cell types for controls, and 119, 135, 129 and 108 cell types for mutants, from n for increasing age of 2, 4, 2 and 2 independent experiments per genotype. Boxplots in b and c show median, interquartile range and 1.5× interquartile range. d , Aggregated expression of 243 core neuronal maturation genes in wild-type C57BL/6J cell types at each age (left) or Trpc2 −/+ or Trpc2 −/− cell types at P10 (right). P value indicates results from two-sided t -test, mutant versus control cell types. e , Random-forest classifier approach (Augur) to identify cell types that show differences between Trpc2 −/+ and Trpc2 −/− cell types at P65. Each point represents a single cell type. Cell types with roles in relevant social behaviours affected in Trpc2 −/− adults are shown with coloured dots. AUC, area under the curve.

Article Snippet: Mutant and transgenic strains used in this study were previously published and include (with Jackson Laboratories (RRID:SCR_004633) numbers where applicable): Trpc2 knockout mice (021208; RRID:IMSR_JAX:021208); Cnga2 knockout mice ; Trpm8 knockout mice (008198; RRID :IMSR_JAX:008198); Piezo2 fl (ref. ) (027720; RRID:IMSR_JAX:027720); Piezo2 − (ref. ); Cdx2 - c re ) (009350; RRID:IMSR_JAX:009350); Avil-cre , Gabrb3 fl (ref. ), and Opn5-cre .

Techniques: Mutagenesis, Control, Knock-Out, Functional Assay, Expressing

Journal: Nature

Article Title: Sensory input, sex and function shape hypothalamic cell type development

doi: 10.1038/s41586-025-08603-0

Figure Lengend Snippet: ( a ) Distance between mutant and control sample centroids in PCA space (normalized to intra-genotype distance) for inhibitory clusters. Control samples included both C57BL6/J and control littermates. Sample numbers, left to right columns, mutant/control: 2/8, 4/10, 2/10, 2/8, 2/8, 2/6, 2/7, 2/7, 2/8, 2/8, 2/7. ( b ) Random forest classifier approach (Augur) to identify cell types that show differences between mutant and control samples. Each point represents a single cell type. Number of biological replicates as described in ( a ). ( c ) Mutant-control PCA distance for all clusters in Trpc2 P10 experiments, split according to sub-region, and ordered from most to least affected. Data from n = 4 biological replicates per genotype. ( d ) UMAP showing identification of visual cortex neuronal subtypes in Trpc2 mutants and controls (pooled) at P10. ( e ) Random forest classifier (Augur) for replication of Trpc2 P10 POA (left) and visual cortex (right) from the same brains. Cells pooled from n = 2 biological replicates per genotype. ( f ) Weight of Trpc2−/− pups and Trpc2−/+ littermates at P7 to P14. n = 41 (Trpc2−/+) and 30 (Trpc2−/−). Error bars show standard error of the mean. ( g ) Latency for mothers to retrieve Trpc2−/+ or Trpc2−/− P9-10 pups when given a choice between the two. n = 48 (Trpc2−/+) and 48 (Trpc2−/−). ( h ) Latency for Trpc2−/+ or Trpc2−/− P13-14 pups to first contact their anesthetized mother. n = 26 (Trpc2−/+) and 22 (Trpc2−/−). ( i ) Latency for Trpc2−/+ or Trpc2−/− P11-12 pups to first contact with littermates. n = 36 (Trpc2−/+) and 20 (Trpc2−/−). ( j ) Latency for Trpc2−/+ or Trpc2−/− P11-12 pups to enter behavioral quiescence. n = 48 (Trpc2−/+) and 30 (Trpc2−/−). ( k ) Ultrasonic vocalizations emitted by isolated Trpc2−/+ or Trpc2−/− P13-14 pups, placed in a clean cage for 30 min at room temperature or with a heating pad underneath. Boxed portion quantified in ( l ). n = 22 (Trpc−/+), 21 (Trpc2−/−). Error bars show standard error of the mean. ( l ) Difference in the number of vocalizations between room temperature and heating pad conditions for each pup, at 20-30 min post-isolation (boxed portion in ( k )). In all panels, boxplots show median, interquartile range, and 1.5 * interquartile range. For panels ( g-l ), p-value indicates results from two-sided t-test.

Article Snippet: Mutant and transgenic strains used in this study were previously published and include (with Jackson Laboratories (RRID:SCR_004633) numbers where applicable): Trpc2 knockout mice (021208; RRID:IMSR_JAX:021208); Cnga2 knockout mice ; Trpm8 knockout mice (008198; RRID :IMSR_JAX:008198); Piezo2 fl (ref. ) (027720; RRID:IMSR_JAX:027720); Piezo2 − (ref. ); Cdx2 - c re ) (009350; RRID:IMSR_JAX:009350); Avil-cre , Gabrb3 fl (ref. ), and Opn5-cre .

Techniques: Mutagenesis, Control, Isolation